SAW Instruments GmbH

sam^®5

Sam^®5 is a small footprint, benchtop biosensor which uses surface acoustic waves to detect and display biomolecular interactions and kinetics in real time. It is a single syringe fluid-based system and guides the aqueous sample for analysis over a Sensor chip support surface. Depending on the assay and attachment chemistries (see separate web page on chips and chemistries), the sensor surfaces can be covered with different ligands. This way, the analysed substances are specifically bound to the sensor surface. The sensor chips used on sam5 contain five independent sensors, providing five measuring channels. Injected sample flows meanders sequentially across the five sensors and thus a single sample can be characterized for up to five different bound analytes.

The central detection unit of sam5 holds a single sensor chip on a sample holder and is temperature controlled. In combination with the autosampler, a complete series of unattended measurements can be set up and executed e.g. overnight or over the weekend. The autosampler has two SBS footprint positions – either rack for 48 vials or 96 well plate.

A family of Sam^®5 products have been developed to reflect the different needs of groups in academia and industry.

Video: sam^®5 biosensor featured on SelectScienceTV

	sam®5 BLUE	sam®5 GREEN
sam®5 and Autosampler Footprint (W x D x H)	30 cm x 51 cm x 55 cm	30 cm x 51 cm x 55 cm
User/Analysis software	Method writing – SequenceMaster® Data collection – SensMaster® Data analysis– FitMaster®	Same (but with usage tracking of the chip)
Sensor chip	5 channels	5 channels
Re-useable chips	Yes – gold and other chemistries User can re-coat	Limited Functionalized chemistries
Chip pricing relative unit	Higher – ability to re-coat	Cheaper – coatings have a finite life
Chip quality	If self-made surfaces, variability	Highly reproducible with batch QC
Chip holder	User loads chip using tweezers into universal chip holder	Chip comes conveniently mounted and fixed in disposable chip holder
Fluidic cell replacement	Recommended to change once per 6 months	Software prompt for change based on tracking of chip usage time
Typical customer groups	Academics Multi users	Industry Dedicated groups with routine assays
Key benefit	Maximum flexibility	Repeatability and Precision
	sam®5 BLUE is already well established in universities and teaching laboratories where it is the ideal tool for methods development. It provides maximum versatility and flexibility for a diverse group of users with different needs and who want a system which is easy to use.	sam®5 GREEN builds upon the proven sam®5 platform. It has been developed with industrial customers in mind who are typically focused on a smaller, defi- ned set of applications and workflows. They require high reproducibility and consistency afforded by batch-control- led, pre-functionalized chips and an on-line audit trail of their chip.

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